How to set the electroporation system? And how to choose optimum electroporation parameters?
The electroporation system is mainly based on the type of cells and the type of electrodes used. The common electroporation system of 96-well plate electrode needles is 1×106 cell/(3-5) μg plasmid/100 μl buffer.
The optimum electroporation parameters are mainly related to the type of cells, the selected electrotroporation buffer and the selected plasmid. For common cells, there are many optimum electroporation parameters you can call directly from our "Cell Databank".